SEGURA
RESEARCH OVERVIEW
Research in my laboratory over the past nine years has focused on the generation of hydrogel biomaterials to support the formation of a reparative niche within diseased or injured sites that can block or prevent inhibitory signals from dominating the repair process, while providing pro-repair signals that can guide new tissue formation. The goal of our approach is to use engineered materials to “unlock” the regenerative capacity of damaged or diseased tissue to promote repair. The premise of our approach is that all tissues in the body have the capacity to repair through local stem or progenitor cells, but that due to unfavorable environmental conditions during the normal healing process they are not able to do so. Our general strategy has been to combine our biomaterials engineering with designing materials that promote the formation of a space filling vascular plexus that could serve as part of a reparative niche directly at the wound site. The idea is that this vascular plexus would lay the groundwork for the recruitment of endogenous stem cells located in the local tissue surrounding the damaged area and generate an environment that would foster repair rather than scaring.  Current projects focus on: •The design of hydrogel biomaterials that allow rapid cellular infiltration and vascularization upon implantation. •Understanding how cell-material interactions can be engineered to guide vascular patterning.  •The design of controlled delivery systems for genes, proteins and small molecules to be introduced into our hydrogel materials. •We utilize skin wound healing models in normal and diabetic animals and stroke models to test our hypotheses.
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CONTACT INFORMATION Mailing Address Prof. Tatiana Segura 420 Westwood Plaza 5531 Boelter Hall  Los Angeles, CA 90095 (310) 794-2248   (Lab)                (310) 206 3980 (office) tsegura at ucla.edu
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Former Undergraduate StudentsZheyu Zhang, MCDB, Start date: 12/2013 Tiffany Huang, Chemical and Biomolecular Engineering, Start date: 3/2013 Ricki Chairil, Chemical and Biomolecular Engineering, Start date: 7/2013 Gerard Nash Gaw, Chemical and Biomolecular Engineering, Start date: 7/2013 Angela Wong, Chemical and Biomolecular Engineering, Start date: 7/2012 Tiffany Luu, Biology, Start date: 12/2013 Rohan Kapre, Chemical and Biomolecular Engineering, Start date: 9/2013 HoMing Yim, Chemical and Biomolecular Engineering, Start date: 9/2013 Denny Ng, Chemical and Biomolecular Engineering, Start date: 6/2013 Alphonse Liu, Chemistry, Start date: 4/2013 Maghana Kumar, CBE January 2012-Dec 2012 Shannon Stephens, CBE June 2011-June 2013 Chris Rodman, CBE June 2011-June 2013 Jacob Borrajo, CBE April 2011-June 2013 Fan Wu, CBE Jan 2012-June 2012 Ariana Thacker, CBE June 2010-Dec2011 Ryan Bello, CBE June 2011-Dec 2011 Maha Rahhim, CBE, 2008-2010 Maricela Maldonado, CBE, June 2009-June 2011 Clayton Lin, CBE, June 2010-June 2011 Sarah Kaushal, CBE, Oct 2010-June 2011 Kaajal Bahetti, BE, April 2009-Dec 2010 Frank Cannova, CBE, Sept 2010-Dec 2010 Warren Kadoya, CBE, June 2008-Sept 2010 Robert Hanusa, CBE, June 2008-Dec 2008 Chadwick Shrum, BE, April 2009-June 2009 Amie Carrervo, CBE, Sept 2009-Nov 2009 Zenas Han, CBE, Sept 2009-Sept 2010 Tony Wu, CBE, Jan 2008-June 2010 Jonathan Pan, CBE, March 2008-June 2008 Marie Sutton, CBE, June 2007-June 2008 Jamie Peng, CBE, Oct 2007-March 2008 Jennifer Lara, CBE, Summer 2007 Eleana Manousiouthakis, RPI, BE, Summer 2008 and Summer 2010 Luz Trejo, Neuroscience UCLA Former High School Interns Owen Leddy, Wildwood School, February 2014-August 2014  Zach Irwin, Wildwood School, February 2012-August 2012 Alan, Harvard Westlake February 2012-August 2012 Charlie Steingard, Wildwood School, August 2010-Sept 2011 Andrea Dominguez, Francisco Bravo Medical Magnet High School, HSSEAS summer program Jonah Shwartz, Wildwood School, March 2010-June 2010 Dashel Thompson, WIldwood School, Sept 2009-March 2010 Nikki Koss, Wildwood School, SEGURAS! Summer program, 2010 Caleb Shields, Wildwood School, Aug 2008-Mar 2009 Irene Manousiouthakis, Harvard-Westlake High School, Summer 2009 Brenda Salas, Los Angeles High School of the Arts, SEGURAS! Summer program, 2009 Sayoni Saha, Whitney High School, SEGURAS! Summer program, 2009 Kaley Leshem, Wildwood School, SEGURAS! Summer program, 2009 Carly Goldblatt, Wildwood School, SEGURAS! Summer program, 2009 Cynthia Santos, Los Angeles High School of the Arts, SEGURAS! Summer program, 2008 Fiona Bracho-Perez, Wildwood School, SEGURAS! Summer program, 2008 Lily Alan, Wildwood School, SEGURAS! Summer program, 2008 Cara Rubenstein, Wildwood School, SEGURAS! Summer program, 2008 Saly Soltero, Saint Matthias High School, SEGURAS! Summer program, 2007 Kaajal Bahetti, Whitney High School, Summer 2007, HSSEAS summer program EndFragment
Hydrogels for Gene deliveryOur laboratory has worked extensively to achieve efficient local non-viral gene transfer by incorporating plasmid DNA into hydrogel biomaterials that are later implanted at diseased tissue sites. We have design methods to incorporate non-viral vectors into hydrogel biomaterials without aggregation (a common problem) and used these scaffolds for in vitro and in vivo gene delivery to promote regeneration.  In addition, we have determined that mechanical  properties,  ECM interactions,  and polyplex properties  can modulate non-viral gene transfer from hydrogel biomaterials and that the biological mechanism of gene transfer to cells when they are seeded inside  a  biomaterial  is  different  to  the  mechanism  when  cells  are  seeded  on  standard  tissue  culture  plastic. Selected Publications1.Lei Y, Huang S, Sharif-Kashani P, Chen Y, Kavehpour P, Segura, T. Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds. Biomaterials. 2010 Dec;31(34):9106-16.  PubMed PMID: 20822811; PubMed Central PMCID: PMC3017740.2.Gojgini S, Tokatlian T, Segura T. Utilizing cell-matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels. Mol Pharm. 2011 Oct 3;8(5):1582-91. PubMed PMID: 21823632; PubMed Central PMCID: PMC4104282.3.Dhaliwal A, Oshita V, Segura T. Transfection in the third dimension. Integr Biol (Camb). 2013 Oct;5(10):1206-16. PubMed PMID: 23929354; PubMed Central PMCID: PMC3798060.4.Tokatlian T, Cam C, Segura T. Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice. Biomaterials. 2014 Jan;35(2):825-35. PubMed PMID: 24210142; PubMed Central PMCID: PMC3941464. VEGF Presentation and Therapeutic Angiogenesis Before the studies in our laboratory were conducted, it was known that the implantation of hydrogel scaffolds with bound VEGF (electrostatic or covalently bound) results in vessels that are more highly branched, perfused and mature than when soluble VEGF is encapsulated in the hydrogel. Moreover, although it was (and still is) common practice in bioengineered materials to covalently bind bioactive signals to the matrix, little is done to know the biological ramifications of such immobilization. In these studies we design a system to study cell activation by covalently bound or electrostatically bound ligands. In particular, we studied the role of VEGF- A165 presentation (soluble, electrostatically bound, covalently bound or multivalent) on endothelial cell activation. Our work in this area has introduced technology for the study of signal transduction of bound ligands that could directly compare the signal transduction by soluble ligands. In our approach, we fist grow the cells on PDMS (plastic) sheets and then either expose them to soluble VEGF or flip them on a surface that contained the bound ligand (e.g. VEGF). This way the cells are at the same confluence and “state” before being exposed to VEGF. Using this method we found that covalently bound VEGF can induce the phosphorylation of VEGFR-2 and that release of the VEGF ligand was not necessary. In fact the ligand stays behind on the surface and can induce the phosphorylation of VEGFR-2 on a new set of cells. Further, we found that VEGFR-2 phosphorylation by covalently bound VEGF led to differential signaling than that phosphorylated by soluble VEGF with covalently bound VEGF inducing more Y1214 and p38 activation and soluble VEGF inducing more Y1175 and p42/44 activation. Similar results were found by collagen I bound VEGF-A. Our work with multivalent VEGF also demonstrates differential activation compared to monovalent VEGF, showing that activating endothelial cells with VEGF clusters results in enhanced branching.  Selected publications1.Anderson SM, Chen TT, Iruela-Arispe ML, Segura T. The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Biomaterials. 2009 Sep;30(27):4618-28.  PubMed PMID: 19540581; PubMed Central PMCID: PMC2826152.2.Anderson, S.M., Siegman, S.N., and Segura, T*., "The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching" Biomaterials, 2011. 32(30): p. 7432-7443. PMCID: PMC3601829.3.Anderson, S.M., Shergill, B., Barry, Z.T., Manousiouthakis, E., Chen, T.T., Botvinick, E., Platt, M.O., Iruela-Arispe, M.L., and Segura, T*., "VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF" Integrative biology : quantitative biosciences from nano to macro, 2011. 3(9): p. 887-896. PMCID: PMC3621282. Controlled Protein Delivery Based on Enzyme Responsive Nanocapsules The goal of this research is to deliver growth factors in a dormant protected form that could become activated by the protease wound microenvironment   in a controlled manner.  We have demonstrated that functional   proteins including   growth factors can be encapsulated within degradable nanocapsules using in situ radical polymerization.  The approach is to first surface absorb a monomer using charge interactions (negative surface protein and positive monomer), mix in neutral monomer, mix in a degradable crosslinker and start the polymerization through the addition of radical initiators. The effect of this process is the polymerization of a polymeric network around the protein.  By controlling the concentration of the protein, monomers and crosslinker, nanoparticles (nanocapsules) can be generated that are stable at physiological conditions.  The choice of the crosslinker is critical for inducing release of the growth factor cargo in the extracellular space such that the growth factor can interact with surface receptors. We chose a plasmin protease degradable crosslinker that can release the cargo at wound sites. Last, to modulate growth factor release rate from the nano capsules, we mixed degradable and non-degradable crosslinker and demonstrated that the ratio of degradable  and non-degradable  crosslinker can indeed modulate release rate.  1.Yan, M., Du, J., Gu, Z., Liang, M., Hu, Y., Zhang, W., Priceman, S., Wu, L., Zhou, Z.H., Liu, Z*., Segura, T*., Tang, Y*., and Lu, Y*., "A novel intracellular protein delivery platform based on single-protein nanocapsules" Nature nanotechnology, 2010. 5(1): p. 48-53. PubMed PMID: 19935648.  2.Wen, J., Anderson, S.M., Du, J., Yan, M., Wang, J., Shen, M*., Lu, Y*., and Segura, T*., "Controlled Protein Delivery Based on Enzyme-Responsive Nanocapsules" Advanced materials, 2011. Advanced materials, 2011. Oct 18;23(39):4549-53 PubMed PMID: 21910141; PubMed Central PMCID: PMC3263975. Biomaterials for stem cell transplantation and differentiationAlthough stem/progenitor transplantation  after stroke leads to improved functional recovery, direct delivery of the cells has met with limited cell survival, and this appears to occur because of cell stress and death of the transplanted cells in the hostile environment of the post-stroke brain. We have begun to study the design of hyaluronic acid injectable hydrogel scaffolds as a vehicle for stem cell transplantation into the stroke cavity to promote their survival and differentiation.  After optimizing the process of transplantation (injection speed, needle gauge and final gel stiffness) we transplanted iPS-NPCs into the infarct cavity of stroked immunodeficient NSG mice with or without a hydrogel and  found  that  delivering  neural  progenitors  within  our  hydrogel  promoted  increased differentiation of these cells to immature neurons (CDX positive), compared to delivering cells in saline. As mentioned transplantation of iPS-NPCs within a hydrogel matrix resulted in differentiation to immature neurons but no mature neurons were observed. Thus, we are further engineering the HA hydrogel to promote differentiation towards mature neurons. We utilized a multifactorial design of experiments  (DOE) approach to systematically modulate three bioactive signal motifs (RGD, YIGSR, IKVAV) to determine their individual and collective effects on encapsulated  iPS-NPC survival.   After three optimization rounds, the final iteration formulation was further investigated for its ability to promote differentiation of the NPCs. Compared to cells plated in 2D and un-optimized hydrogels,  more  mature  neurons  were  observed  in  the  optimized  hydrogels.  Un-optimized hydrogels included RGD only and equimolar amounts of all three peptides (what is commonly done). Overall, our current findings illustrate the benefits of using both the DOE approach in multi-factor systems and culturing NPCs in a three-dimensional hydrogel. We are currently following with in vivo experiments.    1.Zhang, J., Tokatlian, T., Zhong, J., Ng, KT Q., Patterson, M., Lowry, S.W., Carmichael, S.T. Segura, T.* “Physically Associated Synthetic Hydrogels with Long-Term Covalent Stabilization for Cell Culture and Stem Cell Transplantation” Advanced Materials 2011 Nov 16;23(43):5098-103 PubMed PMID:21997799; PubMed Central PMCID: PMC3242734.  2.Lam J, Carmichael ST, Lowry WE, Segura T. Hydrogel Design of Experiments Methodology to Optimize Hydrogel for iPSC-NPC Culture. Adv Healthc Mater. 2014 Nov 5;PubMed PMID: 25378176.  3.Lam J, Lowry WE, Carmichael S, Segura T. Delivery of iPS-NPCs to the Stroke Cavity within a Hyaluronic Acid Matrix Promotes the Differentiation of Transplanted Cells. Adv Funct Mater. 2014 November 26; 24(44):7053. EndFragment
Dr. Don Griffin Postdoc Fellow Ph.D, Bioengineering UCLA Design of microporous hydrogels for tissue healing.
Eunwoo (Chris) Nam, Chemical and Biomecular Engineering
Dr. Weixian Xi Postdoc Fellow Ph.D, Chemical Engineering University of Colorado, Boulder Design of microporous hydrogels for tissue healing.
Patrick Yang, Chemical and Biomecular Engineering
Sandy Li Graduate Student BS.  MS UCLA Bioengineering Nanocapsules for siRNA delivery VEGF presentation and therapeutic angiogenesis
James Lin, Psychobiology
Norman Truong Graduate Student BS, Rice University Hydrogels for local gene delivery and therapeutic angiogenesis
Melisa Rungcharoenporn, Chemical and Biomecular Engineering
Yichi (Steven) Zhang, Chemical and Biomecular Engineering
Elias Sideris Graduate Student BS, Bioengineering University California, Berkeley Design of microporus hydrogels for local gene delivery and brain repait.
Zhiyuan (William) Liu, Chemical and Biomecular Engineering
Adrian Siew, Chemical and Biomecular Engineering
Nicole Darling Graduate Student BS, Chemical Engineering Universigy Colorado, Boulder Design of microporous hydrogels for tissue regeneration.
Ragini Bahttacharya, Bioengineering
Ben Krahenbuhl, Chemical and Biomecular Engineering
Principle Investigator
Shruti Sharma
Undergraduate Students
Ingrid Channa, Psychobiology
Dr. Tatiana Sequra, PhD Associate Professor Chemical and Biomedical Engineering Department tsegura@ucla.edu  
Xin Yu Mao, Chemical and Biomecular Engineering
Donovan Tokuyama
Postdoctoral Scholars
James Papadopoulos, Chemical and Biomecular Engineering
High School Students
Victor Oshita Graduate Student BS, Chemical Engineering UCLA Design of hydrogels for stroke treatment and nanocapsules for growth factor delivery 
Doctoral Students
Evan Chen, Chemical and Biomecular Engineering
Master's Students
Dr. Lina Nih Postdoc Fellow and Lab Manager Ph.D, Neuroscience Angiogenesis and Translational Research Center, Paris, Texas Design of hydrogels for smoke treatment  
Dr. Sasha Cai Lesher-Perez Postdoc Fellow Ph.D, Bioengineering University of Michigan Microfluidic approaches for the study of brain repair.
51. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Siegman S, Truong NF, Segura T.Acta biomaterialia. 2015; 28:45-54. NIHMSID: NIHMS728286PubMed [journal]PMID: 26391497 PMCID: PMC4648651 50. Systematic evaluation of natural scaffolds in cutaneous wound healing. Cam C, Zhu S, Truong NF, Scumpia PO, Segura T.Journal of materials chemistry. B, Materials for biology and medicine. 2015; 3(40):7986-7992. NIHMSID: NIHMS730178PubMed [journal]PMID: 26509037 PMCID: PMC461832249. Imine Hydrogels with Tunable Degradability for Tissue Engineering. Boehnke N, Cam C, Bat E, Segura T, Maynard HD.Biomacromolecules. 2015; 16(7):2101-8. NIHMSID: NIHMS721726PubMed [journal]PMID: 26061010 PMCID: PMC4583069   48. Accelerated wound healing by injectable microporous gel scaffolds assembled from annealed building blocks. Griffin DR, Weaver WM, Scumpia PO, Di Carlo D, Segura T.Nature materials. 2015; 14(7):737-44. NIHMSID: NIHMS721705PubMed [journal]PMID: 26030305 PMCID: PMC461557947. Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control. Zhu S, Nih L, Carmichael ST, Lu Y, Segura T.Advanced materials (Deerfield Beach, Fla.). 2015; 27(24):3620-5. NIHMSID: NIHMS699217PubMed [journal]PMID: 25962336 PMCID: PMC4633528 46. Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model. Tokatlian T, Cam C, Segura T.Advanced healthcare materials. 2015; 4(7):1084-91. NIHMSID: NIHMS666500PubMed [journal]PMID: 25694196 PMCID: PMC4433401 45. Hydrogel design of experiments methodology to optimize hydrogel for iPSC-NPC culture. Lam J, Carmichael ST, Lowry WE, Segura T.Advanced healthcare materials. 2015; 4(4):534-9. NIHMSID: NIHMS671962PubMed [journal]PMID: 25378176 PMCID: PMC4384641 44. Delivery of iPS-NPCs to the Stroke Cavity within a Hyaluronic Acid Matrix Promotes the Differentiation of Transplanted Cells. Lam J, Lowry WE, Carmichael ST, Segura T.Advanced functional materials. 2014; 24(44):7053-7062. NIHMSID: NIHMS671966PubMed [journal]PMID: 26213530 PMCID: PMC4512237   43. Gold-nanocrystal-enhanced bioluminescent nanocapsules. Du J, Jin J, Liu Y, Li J, Tokatlian T, et al.ACS nano. 2014; 8(10):9964-9.PubMed [journal]PMID: 25243486 42. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Nowak-Sliwinska P, Segura T, Iruela-Arispe ML.Angiogenesis. 2014; 17(4):779-804. NIHMSID: NIHMS721735PubMed [journal]PMID: 25138280 PMCID: PMC4583126 41. Hydrogel-based nanocomposites of therapeutic proteins for tissue repair. Zhu S, Segura T.Current opinion in chemical engineering. 2014; 4:128-136. NIHMSID: NIHMS554282PubMed [journal]PMID: 24778979 PMCID: PMC4000039   40. Design of cell-matrix interactions in hyaluronic acid hydrogel scaffolds. Lam J, Truong NF, Segura T.Acta biomaterialia. 2014; 10(4):1571-80. NIHMSID: NIHMS510478PubMed [journal]PMID: 23899481 PMCID: PMC3903661 39. Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation. Griffin DR, Borrajo J, Soon A, Acosta-Vélez GF, Oshita V, et al.Chembiochem : a European journal of chemical biology. 2014; 15(2):233-42. NIHMSID: NIHMS577377PubMed [journal]PMID: 24399784 PMCID: PMC4024472 38. Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice. Tokatlian T, Cam C, Segura T.Biomaterials. 2014; 35(2):825-35. NIHMSID: NIHMS532565PubMed [journal]PMID: 24210142 PMCID: PMC3941464 37. Chemical sintering generates uniform porous hyaluronic acid hydrogels. Cam C, Segura T.Acta biomaterialia. 2014; 10(1):205-13. NIHMSID: NIHMS530792PubMed [journal]PMID: 24120847 PMCID: PMC3941472 36. Matrix-based gene delivery for tissue repair. Cam C, Segura T.Current opinion in biotechnology. 2013; 24(5):855-63. NIHMSID: NIHMS471511PubMed [journal]PMID: 23680305 PMCID: PMC3770770   35. Transfection in the third dimension. Dhaliwal A, Oshita V, Segura T.Integrative biology : quantitative biosciences from nano to macro. 2013; 5(10):1206-16. NIHMSID: NIHMS516398PubMed [journal]PMID: 23929354 PMCID: PMC3798060 34. The modulation of MSC integrin expression by RGD presentation. Lam J, Segura T.Biomaterials. 2013; 34(16):3938-47. NIHMSID: NIHMS445396PubMed [journal]PMID: 23465825 PMCID: PMC3650837   33. Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles. Pannier AK, Segura T.Methods in molecular biology (Clifton, N.J.). 2013; 948:149-69.PubMed [journal]PMID: 23070769 32. Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs. Tokatlian T, Cam C, Siegman SN, Lei Y, Segura T.Acta biomaterialia. 2012; 8(11):3921-31. NIHMSID: NIHMS475834PubMed [journal]PMID: 22820309 PMCID: PMC3678541 31. Biocompatible hydrogels by oxime Click chemistry. Grover GN, Lam J, Nguyen TH, Segura T, Maynard HD.Biomacromolecules. 2012; 13(10):3013-7. NIHMSID: NIHMS407745PubMed [journal]PMID: 22970829 PMCID: PMC3474544 30. Cellular cytoskeleton dynamics modulates non-viral gene delivery through RhoGTPases. Dhaliwal A, Maldonado M, Lin C, Segura T.PloS one. 2012; 7(4):e35046.PubMed [journal]PMID: 22509380 PMCID: PMC3324413 29. Physically associated synthetic hydrogels with long-term covalent stabilization for cell culture and stem cell transplantation. Zhang J, Tokatlian T, Zhong J, Ng QK, Patterson M, et al.Advanced materials (Deerfield Beach, Fla.). 2011; 23(43):5098-103. NIHMSID: NIHMS333069PubMed [journal]PMID: 21997799 PMCID: PMC3242734 28. Controlled protein delivery based on enzyme-responsive nanocapsules. Wen J, Anderson SM, Du J, Yan M, Wang J, et al.Advanced materials (Deerfield Beach, Fla.). 2011; 23(39):4549-53. NIHMSID: NIHMS333076PubMed [journal]PMID: 21910141 PMCID: PMC3263975 27. Utilizing cell-matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels. Gojgini S, Tokatlian T, Segura T.Molecular pharmaceutics. 2011; 8(5):1582-91. NIHMSID: NIHMS592167PubMed [journal]PMID: 21823632 PMCID: PMC4104282 26. The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching. Anderson SM, Siegman SN, Segura T.Biomaterials. 2011; 32(30):7432-43. NIHMSID: NIHMS306388PubMed [journal]PMID: 21783250 PMCID: PMC3601829 25. VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF. Anderson SM, Shergill B, Barry ZT, Manousiouthakis E, Chen TT, et al.Integrative biology : quantitative biosciences from nano to macro. 2011; 3(9):887-96. NIHMSID: NIHMS333074PubMed [journal]PMID: 21826315 PMCID: PMC3621282 24. Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery. Lei Y, Rahim M, Ng Q, Segura T.Journal of controlled release : official journal of the Controlled Release Society. 2011; 153(3):255-61. NIHMSID: NIHMS271832PubMed [journal]PMID: 21295089 PMCID: PMC3138902   23. Evolving the use of peptides as components of biomaterials. Collier JH, Segura T.Biomaterials. 2011; 32(18):4198-204. NIHMSID: NIHMS384245PubMed [journal]PMID: 21515167 PMCID: PMC3389831   22. Protein-polymer nanoparticles for nonviral gene delivery.  Zhang J, Lei Y, Dhaliwal A, Ng QK, Du J, et al.Biomacromolecules. 2011; 12(4):1006-14.PubMed [journal]PMID: 21323308 21. Clustered Arg-Gly-Asp peptides enhances tumor targeting of nonviral vectors. Ng QK, Su H, Armijo AL, Czernin J, Radu CG, et al.ChemMedChem. 2011; 6(4):623-7. NIHMSID: NIHMS271999PubMed [journal]PMID: 21442757 PMCID: PMC3079200 20. The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels. Lei Y, Gojgini S, Lam J, Segura T.Biomaterials. 2011; 32(1):39-47. NIHMSID: NIHMS239296PubMed [journal]PMID: 20933268 PMCID: PMC3004019 19. Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds. Lei Y, Huang S, Sharif-Kashani P, Chen Y, Kavehpour P, et al.Biomaterials. 2010; 31(34):9106-16. NIHMSID: NIHMS229750PubMed [journal]PMID: 20822811 PMCID: PMC3017740 18. Protease degradable tethers for controlled and cell-mediated release of nanoparticles in 2- and 3-dimensions. Tokatlian T, Shrum CT, Kadoya WM, Segura T.Biomaterials. 2010; 31(31):8072-80. NIHMSID: NIHMS228267PubMed [journal]PMID: 20688389 PMCID: PMC2945696 17. Quantum-dot-decorated robust transductable bioluminescent nanocapsules. Du J, Yu C, Pan D, Li J, Chen W, et al.Journal of the American Chemical Society. 2010; 132(37):12780-1. NIHMSID: NIHMS231947PubMed [journal]PMID: 20795619 PMCID: PMC2976844 16. Differential uptake of DNA-poly(ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces. Dhaliwal A, Maldonado M, Han Z, Segura T.Acta biomaterialia. 2010; 6(9):3436-47. NIHMSID: NIHMS193814PubMed [journal]PMID: 20371304 PMCID: PMC2910190 15. Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds. Lei Y, Ng QK, Segura T.Microscopy research and technique. 2010; 73(9):910-7. NIHMSID: NIHMS220048PubMed [journal]PMID: 20232458 PMCID: PMC2930112 14. siRNA applications in nanomedicine. Tokatlian T, Segura T.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology. 2010; 2(3):305-15. NIHMSID: NIHMS592190PubMed [journal]PMID: 20135697 PMCID: PMC4104279 13. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. Chen TT, Luque A, Lee S, Anderson SM, Segura T, et al.The Journal of cell biology. 2010; 188(4):595-609.PubMed [journal]PMID: 20176926 PMCID: PMC2828913 12. A novel intracellular protein delivery platform based on single-protein nanocapsules. Yan M, Du J, Gu Z, Liang M, Hu Y, et al.Nature nanotechnology. 2010; 5(1):48-53.PubMed [journal]PMID: 19935648 11. The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Anderson SM, Chen TT, Iruela-Arispe ML, Segura T.Biomaterials. 2009; 30(27):4618-28. NIHMSID: NIHMS170955PubMed [journal]PMID: 19540581 PMCID: PMC2826152 10. Engineering clustered ligand binding into nonviral vectors: alphavbeta3 targeting as an example. Ng QK, Sutton MK, Soonsawad P, Xing L, Cheng H, et al.Molecular therapy : the journal of the American Society of Gene Therapy. 2009; 17(5):828-36.PubMed [journal]PMID: 19240693 PMCID: PMC2835129 9. DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells.  Lei Y, Segura T.Biomaterials. 2009; 30(2):254-65. NIHMSID: NIHMS79535 8. The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype. Adelöw C, Segura T, Hubbell JA, Frey P.Biomaterials. 2008 Jan;29(3):314-26. Epub 2007 Oct 22.PMID: 17953986  7. RNA interference targeting hypoxia inducible factor 1alpha reduces post-operative adhesions in rats. Segura T, Schmokel H, Hubbell JA.J Surg Res. 2007 Aug;141(2):162-70. Epub 2007 Jun 11.PMID: 17561118 6. Synthesis and in vitro characterization of an ABC triblock copolymer for siRNA delivery. Segura T, Hubbell JA.Bioconjug Chem. 2007 May-Jun;18(3):736-45. Epub 2007 Mar 15.PMID: 17358044  5. Gene delivery through cell culture substrate adsorbed DNA complexes. Bengali Z, Pannier AK, Segura T, Anderson BC, Jang JH, Mustoe TA, Shea LD.Biotechnol Bioeng. 2005 May 5;90(3):290-302.PMID: 15800863 Free PMC Article 4. DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach. Segura T, Chung PH, Shea LD.Biomaterials. 2005 May;26(13):1575-84.PMID: 15522759 Free PMC Article  3. Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern.Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD.Biomaterials. 2005 Feb;26(4):359-71.PMID: 15275810 2. Substrate-mediated DNA delivery: role of the cationic polymer structure and extent of modification. Segura T, Volk MJ, Shea LD.J Control Release. 2003 Nov 18;93(1):69-84.PMID: 14602423 1. Surface-tethered DNA complexes for enhanced gene delivery. Segura T, Shea LD.Bioconjug Chem. 2002 May-Jun;13(3):621-9.PMID: 12009954
Postdoctoral Fellow University of California, Los AngelesChemical and Biomolecular Engineering DepartmentSegura Laboratory Although we do not currently have funding for a Postdoctoral fellow. We welcome applictions from self motivated PhD holders that are willing to apply for fellowshipts.  EndFragment
Master Graduate Research Assistant Open Position AnnouncementUniversity of California, Los AngelesChemical and Biomolecular Engineering DepartmentSegura Laboratory We currently have two open positions for a students interested in obtaining a Research Masters of Science Degree. The topics of the research are 1) gene delivery from hydrogel scaffolds for tissue repair and 2) the design of hydrogels for brain repair. Both projects would be in collabation with other members of the lab. EndFragment
Undergraduate Research Assistant Open Position Announcement University of California, Los Angeles Chemical and Biomelecular Engineering Department Segura Lab Our laboratory is currently looking for undergraduate students to begin work this summer 2016. The position is a volunteer position and will be working closely with a graduate student or postdoctoral fellow mentor. If interested please send the following materials to Prof. Segura and Dr. Lina Nih 1. Unofficial transcript 2. Resume 3. Statement of Purpose. Why do you want to join our team?  
Doctoral Graduate Research Assistant Open Position AnnouncementUniversity of California, Los AngelesChemical and Biomolecular Engineering DepartmentSegura Laboratory We currently have two open positions for graduate students for the 2016-2017 academic year. Please apply through either the Chemical Engineering Department or Bioengineering Department. EndFragment