Contact Information

Mailing Address

Prof. Tatiana Segura

420 Westwood Plaza

5531 Boelter Hall

Los Angeles, CA 90095


Lab

7524 Boelter Hall

(310) 794-2248                 


Office

5532-C Boelter Hall

(310) 206 3980

tsegura@ucla.edu

Brief Bio

Dr. Tatiana Segura is a native of Bogotá, Colombia. She received her diploma from the Escuela Pedagogia Experimental (EPE), an experimental educational school in Bogotá, Colombia. Dr. Segura began her scientific training at the University of California, Berkeley where she received a BS in Bioengineering and a minor in Chemical Engineering. She later obtained her Ph.D in Chemical Engineering at Northwestern University. Dr. Segura pursued postdoctoral research training at the Swiss Federal Institute of Technology in Lausanne, Switzerland. She joined the Chemical and Biomolecular Engineering Department at UCLA in July 2006.

Mailing Address:

420 Westwood Plaza

5531 Boelter Hall

Los Angeles, CA

90095


Phone: (310) 206 3980

email: tsegura@ucla.edu

Research Description

My laboratory is interested in the synthesis of cell instructive materials to be used as environments to study stem cell differentiation and angiogenesis in vitro and to promote regeneration of the brain after stroke or the skin at diabetic wound sites. The long-term goal of my research is to design and engineer hydrogel based biomaterials that contain biological signals that (i) can enhance the wound healing rate of hard to treat wounds through the induction of angiogenesis (e.g. therapeutic angiogenesis of ischemic wounds), (ii) can be used to transplant stem cells to diseased sites, and (iii) can be used to study stem cell differentiation and angiogenesis in vitro. To this aim we have worked on the chemistry necessary to generate hydrogel scaffolds and drug delivery strategies to deliver DNA or proteins from these hydrogels. Our most significant research achievements come from the understanding that ligand presentation has profound implications in how both neural progenitor cells and endothelial cells behave. For example, we found that exact concentration and distribution of ECM mimicking peptides within hydrogels can affect neural progenitor cell differentiation with a multifactorial optimized combination resulting in enhanced differentiation compared to using the standard equimolar concentration of each peptide. Further, we found that vascular endothelial growth factor (VEGF) ligand internalization is not required for VEGFR-2 phosphorylation and that phosphorylation by covalently bound VEGF results in differential endothelial cell activation when compared to soluble VEGF. 


Education

1999        BS            Bioengineering, University of California Berkeley

1999        Minor        Chemical Engineering, University of California Berkeley

2004        Ph.D         Chemical Engineering, Northwestern University

 

Employment

2012 Associate Professor, Chemical and Biomolecular Engineering, University of California, Los Angeles, CA

2006 Assistant Professor, Chemical and Biomolecular Engineering, University of California, Los Angeles, CA

2004 Postdoctoral Fellow, Switzerland Institute of Technology, Lausanne, Switzerland; Principal Investigator: Jeffrey A. Hubbell

2002 Scientist Intern Biomaterials Group Genzyme Corporation Cambridge, MA; Principal Investigator: Bob Miller

1999 Research Associate, Northwestern University, Evanston, IL; Principal Investigator: Lonnie D. Shea


Awards and Honors

2014 Section Editor of Biological Engineering Elsevier Current Opinion in Chemical Engineering

2014 NIH Study Section, Gene and Drug Delivery Panel, ad hoc reviewer

2014 Invited Talk Materials Research Society “HYPER for in situ Cell Manipulation”

2014 Invited Talk Society for Biomaterials “VEGF hotspots induce revascularization of the stroke cavity”

2013 Invited Talk NAVBO meeting “Looks matter: VEGF signaling from Biomaterials”

2013 Invited Talk Materials Research Society meeting “2D vs 3D Gene Transfer”

2013 Invited Talk Gordon Conference Biomaterials and Tissue Engineering “Looks matter: VEGF presentation guides endothelial cell phenotype and angiogenesis.

2013 Invited Talk American Chemical Society spring meeting “Chemoselective patterning as a toll to study vessel sprouting”

2012 Invited Talk Society for Biomaterials New Orleans “Cell Instructive Scaffolds to Guide Angiogenesis”

2012 Invited Talk Materials Research Society “Hydrogels for Stem cell Transplantation”

2012 Invited Talk AICHE Bionanotechnology Plenary Session “Bionanotechnology to Design Cell Instructive Scaffolds”

2012 Invited Talk 6th International Conference on Bioengineering and Nanotechnology “Bionanotechnology to Guide Vessel Sprouting”

2010 Invited Talk Department of Biomedical Engineering, New Innovator’s seminar series, Georgia Institute of Technology, “Gene Transfer in Three Dimensions”

2010 NSF CBET Review Panel, ad hoc reviewer

2009 Invited to give plenary section at the American Society of Gene Therapy Meeting San Diego during the top abstracts plenary session "Clustered Ligands Enhance Targeting of Polyethyleneimine Vectors In Vivo"

2009 American Society of Gene Therapy Outstanding Young Investigator Award, gave plenary talk " Gene Transfer in Three Dimensions"

2008 American Heart Association National Scientist Development Grant (Declined)

2008 National Science Foundation CAREER Award

2008 NSF CBET Review Panel, ad hoc reviewer

2007 NIH Peer Review Committee, SBIR Gene and Drug Delivery Systems, ad hoc reviewer

2006 NSF CBET Review Panel, ad hoc reviewer


10 latest publications

S Zhu and T Segura “Hydrogel-Based Nanocomposites Of Therapeutic Proteins For Tissue Repair” Current Opinion In Chemical Engineering 2014 1;4:128-136.


DR Griffin, A Soon, GF Acosta-Vélez, J Borrajo, V Oshita, N Darling, J Mack, T Barker, ML Iruela-Arispe, and T Segura "Hybrid Photopatterned Enzymatic Reaction (HYPER) platform" ChemBioChem 2014 15(2) 233-242.


T Tokatlian, C Cam, T Segura “Non-Viral DNA Delivery from Porous Hyaluronic Acid Hydrogels in Mice” Biomaterials 2014 35(2) 825-835.


Cam C, Segura, T. “Chemical Sintering Generates Uniform Porous Hyaluronic Acid Hydrogels” Acta Biomaterialia 2014 10(1):205-13.


J Lam, N Truong, T Segura. “Design of Cell-Matrix Interactions in Hyaluronic Acid Hydrogel Scaffolds” Acta Biomaterialia 2014 10(4):1571-80.


A Dhaliwal, V Oshita and T Segura "Transfection in the third dimension" Integrative Biology 2013, 5 (10), 1206 – 1216 (Cover Article).


C Cam and T Segura “Matrix Based Gene Delivery for Tissue Repair” Curr Opin Biotechnol. 2013 24(5):855-63.


J Lam and T Segura The modulation of MSC integrin expression by RGD presentation. Biomaterials. 2013 34(16):3938-47.


AK Pannier, T Segura “Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles” Methods Molecular Biology 2013 948:149-69.


GN Grover†, J Lam†, TH. Nguyen, T Segura,* and HD Maynard* Biocompatible Hydrogels by Oxime Click Chemistry. Biomacromolecules 2012 8;13(10):3013-7 (†co-first author, *co-corresponding author).


Patents

Shea, LD and Segura, T, "Surface Associated Delivery of Genes and Oligonucleotides," US No. 6,890,556

Segura, T., Shea, LD., Bengali, Z., Anderson, B., Pannier, AK., Chung, PC, and Jang, JH. "Controlled Surface-Associated Delivery Of Genes And Oligonucleotides" US No. 7,029,697

Segura, T Hubbell JA, "Triblock Copolymers for Cytoplasmic Delivery of Gene-Based Drugs" 60/686,188

 

Engineering Cell Instructive Materials

Tatiana Segura, Ph.D.